Yes. Wisconsin requires you to be fingerprinted and a background check. This is done through the company that Wisconsin has authorized. You may obtain your fingerprints throughout Wisconsin at Fieldprint locations. Preregistration is required. The fee to be finger printed is $36 (subject to change).
Registration Website (Preregistration required)
Tip: Try to plan your fingerprinting after you pass your exam and immediately before you apply for your license because the fingerprint data is good for only 6 months. See here.